Kuongororwa kweHFRS - Hemorrhagic Fever neRenal Syndrome

Background

Hantaan virus (HV) ndiyo honzeri huru inokonzera Hemorrhagic Fever ine Renal Syndrome (HFRS). HFRS chirwere chepasi rose chezoonotic acute chinotapukira chinoratidzwa nefivha, kubuda ropa, uye kusagadzikana kwetsvo. Chirwere ichi chine kutanga kwakanyanya, kukurumidza kufambira mberi, uye huwandu hwekufa kwakanyanya, zvichiunza njodzi huru kuhutano hweveruzhinji. Makonzo (seApodemus agrarius neRattus norvegicus) ndiwo matura makuru uye mavector eHV. Kutapurirana kuvanhu kunoitika zvakanyanya kuburikidza neaerosolized excreta (weti, tsvina, mate), kubata zvakananga, kana kurumwa nevector. HFRS inogona kuitika mukati megore, uye ruzhinji rwevanhu runotapukira. Zvinoenderana nehuwandu hweWHO, nyika makumi matatu nembiri pasi rese dzakashuma kubuda kweHV, kunyanya kupararira kwakanyanya muEast Asia, Europe, neBalkan.

Antibody Markers Post HV Infection

Kutevera hutachiona hweHV, immune system yemunhu inogadzira masoja ekudzivirira chirwere, kunyanya HV-IgM neHV-IgG.

● Masoja ekudzivirira chirwere eHV-IgM: Anoshanda sechiratidzo chechirwere chepakutanga, chinowanzoitika mukati memazuva echiratidzo chatanga, uye akakosha pakuongororwa kwechirwere.

● Masoja ekudzivirira chirwere eHV-IgG: Anobuda pave paya uye anogona kuramba aripo kweupenyu hwose, zvichiratidza utachiona hwekare kana kupora. Kuwedzera kwakapetwa kana kana kupfuura kweHV-IgG antibody titer pakati peacute uye convalescent serum samples zvakare kuongororwa kwehutachiona hwehutachiona.

Common HV Diagnostic Methods

Nzira dzazvino dzemurabhoritari dzekuonekwa kweHV dzinosanganisira kuparadzaniswa kwehutachiona, PCR, serological ELISA, uye colloidal goridhe immunoassays.

● Hutachiona hwetsika nePCR zvinopa hunyanzvi hwepamusoro asi zvinotora nguva, zvinoda zvehunyanzvi, uye zvinoda nzvimbo dzemurabhoritari dzepamusoro, zvichitadzisa kushandiswa kwavo kwakapararira.

● Micro-immunofluorescence (MIF) inopa chokwadi chakanaka asi inoda fluorescence microscope uye kududzirwa kwenyanzvi, kurambidza kushandiswa kwemaitiro.

● ELISA uye colloidal goridhe assays inogamuchirwa zvakanyanya mumakiriniki ehutano nekuda kwekureruka kwavo, kukurumidza, kunzwisiswa kwepamusoro uye zvakananga, uye nyore kwekuunganidza sampuli (serum / plasma).

Product Performance

Beier Bio's HV-IgM/IgG (ELISA) Assay Features

● Muenzaniso Rudzi: Serum, plasma

● Sample Dilution: Zvese zviri zviviri IgM neIgG assays anoshandisa yepakutanga tsime sampling ine 1:11 dilution (100µl sampuro diluent + 10µl sampu), kugadzirisa mafambiro uye kuderedza basa remushandisi.

● Reagent Yakagadzirira-Ku-Kushandisa: Zvose zvigadziridzo zvakagadzirira kunze kwekushambidza buffer (20 × yakanyanyisa). Ruvara-coded kuti zvive nyore kuzivikanwa

● Maitiro eIncubation: 30 min / 30 min / 15 min; zvizere otomatiki

● Kuona Wavelength: 450 nm ine 630 nm reference

● Coated Strips: 96 kana 48 matsime anogona kuputsika, rimwe nerimwe riine kodhi yezvigadzirwa zvakadhindwa kuti zvionekwe uye zvive nyore.

Beier Bio's HV-IgM/IgG (Colloidal Gold) Assay Features

● Muenzaniso Rudzi: Serum

● Kuona Nguva: Migumisiro mukati memaminitsi e15; hapana mimwe midziyo inodiwa; yakanakira kuongororwa nekukurumidza muvarwere vekunze, emergency, uye vakapararira varwere

● Maitiro: Wedzera 10µl sampuli kumuenzaniso wekadhi rekuedza zvakanaka uchishandisa dropper; dudzira mhinduro mukati memaminitsi gumi nemashanu kusvika makumi maviri

Clinical Performance yeHV-IgM (ELISA), HV-IgG (ELISA), uye HV-IgM/IgG (Colloidal Gold)