Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Iyi kit inoona anti-cyclic citrullinated peptide antibodies (CCP antibodies) mumasampuli emuserum evanhu zvichienderana nenzira isina kunanga, ine yakacheneswa cyclic citrullinated peptide antigens inoshandiswa se antigen yekuputira.

Maitiro ekuyedza anotanga nekuwedzera iyo serum sampu kumatsime ekuita ayo akafanoputirwa neambotaurwa akacheneswa maantigen, achiteverwa nenguva yekunyura. Munguva yeiyi incubation, kana masoja ekudzivirira chirwere eCCP aripo mumuenzaniso, anozonyatso ziva nekusunga kuma cyclic citrullinated peptide antigens akaputirwa pamamicrowells, achigadzira yakagadzikana antigen-antibody complexes. Kuti ave nechokwadi chekururama kwematanho anotevera, zvikamu zvisingabhadharwi mumatsime ekugadzirisa zvinobviswa kuburikidza nekugeza, izvo zvinobatsira kubvisa kukanganisa kunogona kubva kune zvimwe zvinhu mu serum.

Tevere, enzyme conjugates inowedzerwa kumatsime ekuita. Mushure mekuwedzera kwechipiri, aya enzyme conjugates anozonyatso batanidza kune iripo antigen-antibody complexes, ichigadzira yakakura immune system inosanganisira iyo antigen, antibody, uye enzyme conjugate. Kana TMB substrate solution inounzwa muhurongwa, enzyme iri muconjugate inokonzeresa kuita kwemakemikari neTMB substrate, zvichikonzera shanduko yeruvara inooneka. Hukuru hwekuita kweruvara urwu hunoenderana zvakananga nehuwandu hwemasoja ekudzivirira chirwere eCCP aripo mune yekutanga serum sample. Pakupedzisira, microplate reader inoshandiswa kuyera kunyura (A value) yemusanganiswa wekuita. Nekuongorora kukosha uku kwekutora, huwandu hweCCP masoja ekudzivirira chirwere mumuenzaniso hunogona kutariswa nemazvo, zvichipa hwaro hwakavimbika hwekuongororwa kwekiriniki uye kuongororwa.