Anti-Islet Cell (ICA) Antibody ELISA Kit

Tsanangudzo Pfupi

Iyi kit yakagadzirirwa iyo yemhando mu vitro yekuona yeanti-islet cell antibody (ICA) mazinga muserum yevanhu. Mukiriniki, inonyanya kushandiswa sechishandiso chekubatsira chekuongorora Type 1 Diabetes Mellitus (T1DM).

Islet cell antibodies ndeye autoantibodies inotarisa maantigen pamusoro kana mukati mepancreatic islet masero, kunyanya β masero. Kuvapo kwavo kwakabatana zvakanyanya nekukuvara kwe autoimmune kune islet masero, inova yakakosha pathological chimiro cheT1DM. Mumatanho ekutanga eT1DM, kunyange zvisati zvaonekwa zviratidzo zvekiriniki zvakadai se hyperglycemia, ICA inowanzoonekwa mu serum, zvichiita kuti ive yakakosha kutanga kwekudzivirira kwechirwere chechirwere.

Kune vanhu vane nhoroondo yemhuri yechirwere cheshuga kana avo vanoratidza pre-diabetic zviratidzo, kuona ICA mazinga kunobatsira kuongorora njodzi yekuvandudza T1DM. Uyezve, kune varwere vane zvikonzero zvisina kujeka zve hyperglycemia, ICA yekuongorora rubatsiro mukusiyanisa T1DM kubva kune mamwe marudzi echirwere cheshuga, nokudaro kutungamirira kuumbwa kwezvirongwa zvakakodzera zvekurapa. Nekutarisa shanduko mumazinga eICA, inogona zvakare kupa referensi yekuongorora kufambira mberi kweislet cell kukuvara uye kushanda kwematanho ekupindira.

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Principle

Iyi kit inoona islet cell antibodies (ICA) mumaserum emunhu sampuli zvichibva pane isina kunanga nzira, ine akacheneswa islet cell antigens anoshandiswa seyekuputira antigen.

Maitiro ekuyedza anotanga nekuwedzera serum sampuli kune matsime ekuita pre-akaputirwa neantigen, inoteverwa nekufukidzira. Kana ICA iripo mumuenzaniso, inonyatso sunga kune yakavharwa islet cell antigens mumatsime, ichigadzira yakagadzikana antigen-antibody complexes. Zvinhu zvisina kusungwa zvinozobviswa kuburikidza nekugeza kuti zvive nechokwadi chechokwadi chezvinozoitika.

Zvadaro, enzyme conjugates inowedzerwa kumatsime. Mushure mechikamu chechipiri chekuwedzera, aya enzyme conjugates anosunga kune iripo antigen-antibody complexes. Kana TMB substrate solution ikaunzwa, enzyme mune yakaoma inokonzeresa kuita neTMB, zvichikonzera shanduko yeruvara inooneka. Pakupedzisira, muverengi we microplate anoshandiswa kuyera kunyura (A value), iyo inobvumira kutsunga kwemazinga eICA mumuenzaniso zvichienderana nehukuru hwekuita kwemavara.

Product Features

High senitivity, chaiyo uye kugadzikana

Chigadzirwa Specification

Principle	Enzyme yakabatana immunosorbent assay
Type	IndirectNzira
Chitupa	NMPA
Muenzaniso	Serum yevanhu / plasma
Tsanangudzo	48T /96T
Kuchengetedza tembiricha	2-8℃
Sherufu hupenyu	12mwedzi