Anti-Ovarian (AO) Antibody ELISA Kit

Tsanangudzo Pfupi

Ovary ine mazai, zona pellucida, granulosa masero, nezvimwewo, pamatanho ekukura akasiyana. Chikamu chega chega chinogona kukonzera maanti-ovarian antibodies (AoAb) nekuda kweabnormal antigen kutaura. Ovarian antigen spillage inokonzerwa nekukuvara kwe ovarian, kutapukirwa, kana kuzvimba kunogona kukonzera AoAb muvanhu vane immune dysfunction. AoAb inowedzera kukuvadza ovary uye inokanganisa uterine uye placenta mabasa, zvichikonzera kusabereka uye kupfupfudzika.

AoAb yakatanga kuwanikwa muvarwere vane premature ovarian kukundikana (POF) uye yekutanga amenorrhea, yakabatana ne autoimmune reactions. AoAb pakutanga inoderedza kubereka uye pakupedzisira inotungamira mukukundikana kweovarian. Varwere vasina mbereko vane AoAb yakanaka asi pasina POF inogona kutarisana nenjodzi dzePOF dzemberi, zvinoda kuongororwa ovarian reserve.

AoAb positivity yakakwira muvarwere vasina mbereko uye vanobva pamuviri, zvichiratidza hukama hwepedyo. Zvidzidzo zvinoratidza AoAb inokonzeresa kushaya mbereko pane kupfupfudzika. Tsvagiridzo ichangoburwa inoona AoAb muvarwere vazhinji vePCOS, ichikurudzira kuzvimba-induced ovarian kuzvimba uye ma cytokines asina kujairika zvinogona kukonzera PCOS uye kushaya mbereko, izvo zvinoda kumwe kudzidza.

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Principle

Iyi kit inoona maanti-ovarian antibodies (IgG) mumasamples emuserum yevanhu zvichibva nenzira isina kunanga, ine maantigen akacheneswa ovarian membrane anoshandiswa kufanovhara mamicrowell.

Maitiro ekuyedza anotanga nekuwedzera serum sampuli kune antigen-precoated reaction matsime ekuisa. Kana maanti-ovarian antibodies aripo mumuenzaniso, anonyatso sunga kune pre-yakavharwa ovarian membrane antigens mumamicrowells, kugadzira yakagadzikana antigen-antibody complexes. Zvinhu zvisina kusungwa zvinobviswa kuti zvive nechokwadi chekuonekwa.

Tevere, horseradish peroxidase (HRP) -yakanyorwa mbeva inorwisa-munhu IgG masoja ekudzivirira chirwere anowedzerwa kumatsime. Mushure mekuwedzera kwechipiri, masoja ekudzivirira chirwere aneenzayimu aya anosunga masoja ekudzivirira ovarian ari mumaantigen-antibody complexes aripo, achigadzira "antigen-antibody-enzyme label" yakakwana.

Pakupedzisira, TMB substrate solution inowedzerwa. Iyo HRP mune yakaoma inokonzeresa kuita kwemakemikari neTMB, ichigadzira inooneka shanduko yeruvara. Kunwa (A value) kwemhinduro yemhinduro kunoyerwa pachishandiswa microplate reader, uye kuvapo kana kusavapo kweAnti-ovarian antibodies mumucherechedzo kunotariswa zvichienderana nemhedzisiro yekutora.

Product Features

High senitivity, chaiyo uye kugadzikana

Chigadzirwa Specification

Principle	Enzyme yakabatana immunosorbent assay
Type	IndirectNzira
Chitupa	NMPA
Muenzaniso	Serum yevanhu / plasma
Tsanangudzo	48T /96T
Kuchengetedza tembiricha	2-8℃
Sherufu hupenyu	12mwedzi