Anti-Zona Pellucida (ZP) Antibody ELISA Kit

Tsanangudzo Pfupi

Ichi chigadzirwa chakagadzirirwa kutarisisa in vitro yemhando yezona pellucida (ZP) antibody mazinga muserum yevanhu. Iyo zona pellucida, yakakosha extracellular matrix yakatenderedza oocyte, inoita basa rakakosha mukuzivikanwa kwembeu, kusunga, uye kusangana, pamwe nekukura kwekutanga embryo.

Masoja ekudzivirira chirwere eZP masoja ekudzivirira Pavanooneka mumuviri, vanogona kunyatsosunga kune zona pellucida, ichivharira kupindirana kwakajairwa pakati pesperm nemaoocyte, nekudaro zvichitadzisa kubereka. Pamusoro pezvo, vanogona kuvhiringa nzira yekuisirwa kwemazai akagadzirwa, inova chimwe chezvikonzero zvakakosha zve autoimmune infertility.

Mukiriniki, kutariswa uku kunoshanda senzira yekubatsira yekuongorora ye autoimmune infertility. Nekuona huwandu hwemasoja ekudzivirira chirwere eZP muserum yevarwere, inogona kupa ruzivo rwakakosha rwekujekesa zvikonzero zvekusabereka mune vamwe varwere vane zvikonzero zvisingazivikanwi, kubatsira vanachiremba kugadzira zvirongwa zvakanangwa zvekuongorora uye kurapwa.

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Principle

Kiti iyi inocherekedza zona pellucida masoja ekudzivirira chirwere (ZP-Ab) mumasample serum yevanhu zvichibva nenzira isina kunanga, nezona pellucida yakacheneswa inoshandiswa seantigen yekuputira.

Maitiro ekuyedza anotanga nekuwedzera serum sampuli kune matsime ekuita pre-akaputirwa neantigen, inoteverwa nekufukidzira. Kana ZP-Ab iripo mumuenzaniso, inonyatso sunga kune yakavharwa zona pellucida antigen mumatsime, ichigadzira yakagadzikana antigen-antibody complexes.

Zvadaro, enzyme conjugates inowedzerwa kumatsime. Mushure mechikamu chechipiri chekuwedzera, aya enzyme conjugates anosunga kune iripo antigen-antibody complexes. Kana TMB substrate mhinduro ikaunzwa, kuita kwemavara kunoitika pasi pekuita kwekuita kwe enzyme mune yakaoma. Pakupedzisira, microplate reader inoshandiswa kuyera kunyura (A value), iyo inobvumira kutsunga kwemazinga eZP-Ab mumuenzaniso.

Product Features

High senitivity, chaiyo uye kugadzikana

Chigadzirwa Specification

Principle	Enzyme yakabatana immunosorbent assay
Type	IndirectNzira
Chitupa	NMPA
Muenzaniso	Serum yevanhu / plasma
Tsanangudzo	48T /96T
Kuchengetedza tembiricha	2-8℃
Sherufu hupenyu	12mwedzi