Enterovirus 71(EV71) IgM ELISA Kit

Tsanangudzo Pfupi

Enterovirus 71 IgM (EV71-IgM) ELISA kit is enzyme-yakabatana immunosorbent assay yemhando yekuonekwa kweIgM-class masoja ekudzivirira chirwere kuEnterovirus 71 muserum yevanhu kana plasma.Inotarisirwa kushandiswa mumakiriniki marabhoritari kuongororwa uye manejimendi evarwere vane chekuita nehutachiona neEnterovirus 71.

The human enterovirus 71 (EV71), nhengo itsva yeEnteroviridae, ndiyo inowanzokonzera ruoko, tsoka uye chirwere chemuromo (HFMD) uye dzimwe nguva inobatanidza nehutachiona hwehutachiona hwehutachiona.Utachiona huzhinji hunowedzera muna Chikumi naChikunguru.Utachiona hweEV71 hunogona kunge husina zviratidzo kana kuti hunogona kukonzera manyoka uye mapundu.Vana vari pasi pemakore mashanu vari panjodzi huru yekuva nechirwere chakakomba kupfuura vanhu vakuru nevana vakura.

EV71 inotapuriranwa kubva kumunhu ichienda kumunhu kuburikidza nekubata zvakananga uye kuburikidza nekusvibiswa kwemaoko kana zvinhu zvine tsvina ine utachiona.Kubuda mumhino nepahuro, mate kana mvura kubva mumapundu zvinogonawo kuparadzira utachiona.

EV71 inogona kuparadzaniswa uye kuonekwa muhuro uye zvituru zvienzaniso uchishandisa PCR kuyedzwa.Viral RNA yakaonekwa muganda vesicle fluid, ropa uye weti.Kwekutanga uye nyore kuongororwa kunogona kuwanikwa uchishandisa EV71-yakananga serological assays, kusanganisira bvunzo dzakanangana neIgM antibody.

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Principle

Kit iyi inoona Enterovirus 71 IgM antibody (EV71-IgM) muserum yemunhu kana plasma samples, polystyrene microwell strips pre-yakavharwa nemasoja ekudzivirira chirwere anonangidzirwa kumapuroteni emunoglobulin M (anti-μ chain). Mushure mekutanga kuwedzera serum kana plasma specimens kuti zvive akaongororwa, masoja ekudzivirira chirwere eIgM ari mumuenzaniso anogona kutorwa, uye zvimwe zvikamu zvisina kusungwa (kusanganisira chaiwo maIgG antibodies) anozobviswa nekugezwa.Muchikamu chechipiri, HRP (horseradish peroxidase) -conjugated antigens inonyatsoita chete neEV71 IgM antibodies.Mushure mekugeza kubvisa iyo isina kusungwa HRP-conjugate, chromogen solutions inowedzerwa mumatsime.Pamberi pe (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, mushure mekugeza ndiro, iyo TMB substrate yakawedzerwa yekuvandudza kwemavara, uye HRP yakabatana kune yakaoma inokonzeresa maitiro ekugadzira ruvara. kugadzira chinhu chebhuruu, wedzera 50μL yeStop Solution, uye shandura yero.Kuvapo kwekutora kweEV71-IgM antibody mumuenzaniso kwakatemwa nemuverengi wemicroplate.

Product Features

High senitivity, chaiyo uye kugadzikana

Chigadzirwa Specification

Principle	Enzyme yakabatana immunosorbent assay
Type	Capture nzira
Chitupa	CE
Muenzaniso	Serum yevanhu / plasma
Tsanangudzo	48T / 96T
Kuchengetedza tembiricha	2-8℃
Sherufu hupenyu	12 mwedzi