Hepatitis E virus IgM ELISA Kit

Tsanangudzo Pfupi

Chirwere cheHepatitis E IgM Test Cassette inoshandiswa pakuonekwa kwehutano hweHepatitis E Virus IgM masoja ekudzivirira chirwere muvanhu serum, plasma (EDTA, heparin, sodium citrate) kana ropa rose (EDTA, heparin, sodium citrate).Muedzo unofanirwa kushandiswa seyamuro mukuongororwa kwehutachiona hwehepatitis, iyo inokonzerwa neHepatitis E Virus.

Hutachiona hweHepatitis E (HEV) hutachiona hweRNA husina kuputirwa, humwechete hune tambo dzeRNA hunonyanya kutapuriranwa kuburikidza nefecal-oral route, kuwedzerwa ropa uye pamwe naamai-fetus.Kutapukirwa neHEV kunokonzera acute sporadic and epidemic viral Hepatitis uye kunokonzera acute kana subclinical zvirwere zvechiropa zvakafanana neHepatitis A. Kunyange zvazvo kune genotypes ina huru dzeHEV, pane serotype imwe chete.

Hutachiona hweHEV muvanhu hunogadzira masoja ekudzivirira chirwere eIgM, IgA uye IgG.HEV-IgM uye HEV-IgA positivity chiratidzo cheacute kana chazvino hutachiona hweHEV.Kunyangwe anti-HEV-IgM uye anti-HEV-IgA akanaka kune imwechete kana ese ari maviri, anoratidza hutachiona hweHEV huchangobva kuitika.Kuvapo kwehutachiona hweHEV huchangobva kuitika, hwakasanganiswa nekushanda kwechiropa, hunogona kushandiswa kuona kana hutachiona hwacho hwakanyanya kana huchangobva kuitika.Kuvapo kwehutachiona hweHEV muchiropa kunogona kushandiswa kuona kana chirwere ichi chiri acute hepatitis E kana kupora kubva kune acute hepatitis E.

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Principle

Iyi kit inoona hutachiona hweHepatitis E IgM antibody (HEV-IgM) muserum yevanhu kana plasma samples, polystyrene microwell strips pre-yakaputirwa nemasoja ekudzivirira chirwere anonangidzirwa kumunhu immunoglobulin M mapuroteni (anti-μ cheni).Mushure mekutanga kuwedzera serum kana plasma zvienzaniso kuti zviongororwe, masoja ekudzivirira chirwere eIgM ari mumuenzaniso anogona kutorwa, uye zvimwe zvinhu zvisina kusungwa (kusanganisira chaiwo maIgG antibodies) anozobviswa nekugezwa.Muchikamu chechipiri, HRP (horseradish peroxidase) -conjugated antigens inonyatsoita chete neHEV IgM antibodies.Mushure mekugeza kubvisa iyo isina kusungwa HRP-conjugate, chromogen solutions inowedzerwa mumatsime.Pamberi pe (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, mushure mekugeza ndiro, iyo TMB substrate yakawedzerwa yekuvandudza ruvara, uye HRP yakabatana kune yakaoma inokonzeresa maitiro emuvakidzi wemuvara. gadzira chinhu chebhuruu, wedzera 50μl yeStop Solution, uye shandura yero.Kuvapo kwekutorwa kweHEV-IgM antibody mumuenzaniso kwakatemwa nemuverengi wemicroplate.

Product Features

High senitivity, chaiyo uye kugadzikana

Chigadzirwa Specification

Principle	Enzyme yakabatana immunosorbent assay
Type	Capture nzira
Chitupa	CE
Muenzaniso	Serum yevanhu / plasma
Tsanangudzo	48T / 96T
Kuchengetedza tembiricha	2-8℃
Sherufu hupenyu	12 mwedzi